Among the Varanasi temples, Tulsi Manas Temple holds historical and cultural significance in Hinduism. This is because, in the 16th century, the Hindu poet and saint Goswami Tulsidas wrote Ramcharitmanas at this very place. Ramayana, a distinguished epic in Hinduism, was originally written in Sanskrit by Valmiki, approximately between 500 BCE and 100 BCE. As the original Ramayana in Sanskrit was difficult for common people to understand, Tulsidas wrote Ramcharitmanas in simple Hindi.

Tulsi Manas Mandir holds immense religious and cultural importance in Hinduism because Saint Goswami Tulsidas wrote Ramcharitmanas at this very place. Ramcharitmanas is inscribed on the entire wall of the temple, and the recitation of Ramayana continues continuously in the temple.

This temple is constructed in the Hindu architectural style with complete Sangemarmar (marble) material. In the centre of the temple, there are idols of Lord Rama, Mata Janaki, and Lakshmana. On one side are Lord Satyanarayana and Mata Annapurna, and on the other side is Lord Shiva. A special feature of this temple is that the entire Ramcharitmanas is inscribed on the temple walls with beautiful carvings in marble. On the second floor of the temple, there is a statue of Saint Tulsidas. Tulsi Manas Mandir celebrates Ram Navami, Annapurna Puja, and Krishna Janmashtami festivals with great fervour. The first floor of the temple has a library with rare copies of the Ramayana in various languages.